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Gene Therapy for Metastatic Melanoma in Mice Produces Complete Remission

Cancer researchers report a potent anti-tumor gene introduced into mice with metastatic melanoma has resulted in permanent immune reconfiguration and produced a complete remission of their cancer, according to an article to be published in the December 2010 issue of the Journal of Clinical Investigation. ... > full story

Sunday, January 23, 2011

Eukaryotic Cells


 


 
A cell that contains membrane-bound compartments in which specific metabolic activities take place. Most important among these compartments is the nucleus, which houses the eukaryotic cell's . It is DNA this nucleus that gives the eukaryote – literally, "true nucleus" – its name. Eukaryotic organisms also have other specialized, membrane-bounded structures, called organelles, which are small structures within cells that perform dedicated functions. Eukaryotic cells are typically 10 to 100 micrometers across, or about 10 times the size of prokaryotic cells. The set of ideas most favored by biologists to explain how eukaryotic cells first came about is called the endosymbiotic theory. This theory is able to account well for the fact that two of the organelles found in eukaryotic cells, mitochondrias and chloroplasts, have their own DNA that is completely distinct from the DNA housed in the nucleus. According to the endosymbiotic theory, the eukaryotic cell evolved from a symbiotic community of prokaryotic cells. Specifically, the mitochondria and the chloroplasts are what remains of ancient symbiotic oxygen-breathing bacteria and cyanobacteria, respectively, whereas the rest of the cell seems to be derived from an ancestral archaean prokaryote cell.
The origin of the eukaryotic cell was a milestone in the evolution of life. Although eukaryotes use the same genetic code
 and metabolic processes as prokaryotes, their higher level of organizational complexity has permitted the development of truly multicellular organisms. Without eukaryotes, the world would lack mammals, birds, fish, invertebrates, fungi, plants, and complex single-celled organisms. 



 

Prokaryotic Cells





Cells that lack a membrane-bound nucleus are called prokaryotes (from the Greek meaning before nuclei). These cells have few internal structures that are distinguishable under a microscope. Cells in the monera kingdom such as bacteria and cyanobacteria (also known as blue-green algae) are prokaryotes.
Prokaryotic cells differ significantly from eukaryotic cells. They don't have a membrane-bound nucleus and instead of having chromosomal DNA, their genetic information is in a circular loop called a plasmid. Bacterial cells are very small, roughly the size of an animal mitochondrion (about 1-2µm in diameter and 10 µm long). Prokaryotic cells feature three major shapes: rod shaped, spherical, and spiral. Instead of going through elaborate replication processes like eukaryotes, bacterial cells divide by binary fission. Bacteria perform many important functions on earth. They serve as decomposers, agents of fermentation, and play an important role in our own digestive system. Also, bacteria are involved in many nutrient cycles such as the nitrogen cycle, which restores nitrate into the soil for plants. Unlike eukaryotic cells that depend on oxygen for their metabolism, prokaryotic cells enjoy a diverse array of metabolic functions. For example, some bacteria use sulfur instead of oxygen in their metabolism.

DNA translation



In the synthesis or production of proteins, a process of decoding the 'messenger Ribonucleic Acid' or mRNA takes place. It is the first step, and is known as translation. The mRNAs decoded in translation are obtained from a process known as transcription. The translation process takes place in the cell cytoplasm, specifically where the cell organelle, ribosome is present. Translation produces polypeptides as a result of decoding of the mRNA.

Transcription




DNA transcription is a process that involves the transcribing of genetic information from DNA to RNA. The transcribed DNA message is used to produce proteins. DNA is housed within the nucleus of our cells. It controls cellular activity by coding for the production of enzymes and proteins. The information in DNA is not directly converted into proteins, but must first be copied into RNA. This ensures that the information contained within the DNA does not become tainted.

DNA replication


DNA replication begins with the "unzipping" of the parent molecule as the hydrogen bonds between the base pairs are broken. Once exposed, the sequence of bases on each of the separated strands serves as a template to guide the insertion of a complementary set of bases on the strand being synthesized.
The new strands are assembled from deoxynucleoside triphosphates. Each incoming nucleotide is covalently linked to the "free" 3' carbon atom on the pentose (figure) as the second and third phosphates are removed together as a molecule of pyrophosphate (PPi). The nucleotides are assembled in the order that complements the order of bases on the strand serving as the template. Thus each C on the template guides the insertion of a G on the new strand, each G a C, and so on. When the process is complete, two DNA molecules have been formed identical to each other and to the parent molecule.